GenomeLab SNPstream Genotyping System-- SNP genotyping for medium to ultra-high throughput analysis

Beckman GenomeLab SNPstream基因分型系统是一套自动化的多重SNP分析系统，在特制384孔板的每个微孔中可分析12个或48个SNP位点。该系统已广泛应用于遗传图谱标记的绘制、疾病诊断和疾病易感基因的鉴别、药物代谢和药物遗传学、法医鉴定和个体识别等遗传相关研究和应用领域。
准确——采用贝克曼独家专利的单碱基引物延伸SNP(SNP-it)检测技术，准确率高达99%，通过集成的综合软件使质控和数据统计一体化，全程100%质量控制；
快速——灵活的多路线技术与先进的自动化操作保证每天可以处理4,600-3,000,000个基因型，大大加快了您的科研进度；
高转化率——科研工作者常常发现，约有50%的基因或者序列并不适合基于荧光定量PCR的方法，而我们转化率高达85%，完全可以弥补这一缺陷；
超低样本量——每个基因型检测仅需0.04ng的DNA。许多研究者存有多年的、背景资料详实的石蜡包埋块样本，我们也只需薄薄两片，这就是我们无可比拟的天然优势。
可同时进行：10到数百个 SNPs位点检测
48到数千个 DNA 样本
检测物种：人类、老鼠、牛、植物、果蝇、细菌等
检材：血液、组织、细胞培养、口腔黏膜拭子等

SNP genotyping for medium to ultra-high throughput analysis

The GenomeLab SNPstream Genotyping System provides an automated scalable system capable of performing from 4,600 to over 3,000,000 SNP genotypes per day. Combined with multiplexing capabilities, experimental layouts and operational flexibility, this enables you to process a wide range of studies.

Product Features Click to enlarge image 12-plex or 48-plex SNP Genotyping The GenomeLab SNPstream is an automated multiplexed system that can process either 12 or 48 SNPs in each well of an arrayed 384-well plate. This system operates at a consistent cost per sample, regardless of run throughput, allowing users to cost-effectively zero in on research targets with low throughput studies as well as conduct high-throughput analyses. T-2050A: Rapid, Robust and Cost-Effective SNP Analysis with the 48-Plex GenomeLab SNPstream Genotyping System (PDF Format) Click to enlarge image Hardware In a bench-top design, the GenomeLab SNPstream Genotyping System utilizes single-base primer extension technology in a tagged fluorescent assay to deliver an automated, scalable solution for your medium to ultra-high throughput analysis. It comes with reagents, software and instrumentation developed to work optimally together, as well as a two-color CCD-based imager. Integrated Software--Fully Automated Workflow From Raw Data to Genotype Summary Report The SNPstream software suite is a comprehensive set of advanced, easy-to-use tools that work together to provide a seamless workflow, from primer design to 384-well setup, analysis of image data, and genotype calls. Large amounts of data in batch format are handled with minimal user interaction for increased data integrity and ultra-high throughput processing. Seamless Workflow--Up to 72 Plates Unattended SNPware® Reagent Kits SNPware Reagent Kits (12- or 48-plex) Our preformulated, ready-to-use SNPware® Reagent Kits are optimized for effortless and robust single-base primer extension reactions. The kits include ready-to-use reagents for simple addition reactions, all performed in the original PCR* plate. No precipitation or filtration steps are required. SNPware Tag Array Plate Tag Array Method Multiplexed reactions are spatially resolved on a SNPware Tag Array plate. Each of the 384 wells in a plate contain 16 or 52 unique oligonucleotides of a known sequence, or tag. Each tag on the plate is complementary to one of the tags of the 12 or 48 extension primers, plus four controls to ensure accuracy. After transfer to the Tag Array plate, each SNP hybridizes to its complementary tag. SNPs are then identified by their position in the well.

More Info Automation Our assay setup is compatible with either manual operation or different automated solutons. Manual — The GenomeLab SNPstream's assay setup is easily achieved by manual pipetting. Biomek® 3000 — Ideally suited to meet the needs of low- to medium-throughput research applications. Biomek 3000 Laboratory Automation Workstation Biomek NXP — Available in multiple configurations and provides a high degree of flexibility with medium throughput. Biomek NXP Laboratory Automation Workstation Biomek FXP — Offers the highest capacity deck space available and the most flexibility in device integration. Provides total application solutions with either single- or dual-bridge configurations that accommodate a variety of pipetting options. Biomek FXP Laboratory Automation Workstation back to top Autoprimer.com Click to enlarge image Autoprimer.com This innovative, on-line resource takes the uncertainty out of assay design by providing fast, easy-to-use primer design support and tag assignments. Just upload or enter your sequence at www.autoprimer.com and our state-of-the algorithms will instantly design robust, best-fit multiplex PCR and extension primers. By designing panels to work under a single PCR condition as well as extension conditions, Autoprimer.com software takes care of the optimization--so you don't have to. back to top Key Strengths High Fidelity / Accuracy — Single-base primer extension technology delivers accuracy rates of greater than 99%. This patented method — the gold standard — is the most validated technique available. Our system software allows 100% data QC. Sensitive — Only 2 ng of DNA is required per sample for either the 12-plex or 48-plex assay. This equates to 0.04 ng DNA required per genotype. Scalable throughput — Flexible multiplexing and advanced automation provide the capability to process a broad range of 4,600 to over 3,000,000 genotypes per day. Plate-handling robotics increase throughput while providing walk-away operation. Simple — An integrated bar code reader enables accurate sample tracking and reporting. Our standardized reaction conditions and simple steps mean no optimization is necessary. Flexibility — All common plate layouts are supplied enabling analyses. Scalable assay automation, automated data collection, data analysis, and auto QC provides a flexible and fully automated solution from DNA to genotype calls. Cost-effective — By lowering PCR expenses and improving efficiency, the 12-plex 48-plex power built into the GenomeLab SNPstream reduces cost per genotype and per sample. Multiple DNA Sources — Blood, tissue, cell-culture, swab, etc. Multiple Species — Human, mouse, cow, drosphilia. microbes, etc. Service and Assay Support — To help you always get the most out of your Beckman Coulter equipment, we maintain a worldwide reputation for on-line, on-call and on-site support resources. And our experienced applications scientists are available to help you achieve optimum results.

* All trademarks are the property of their respective owners. Where applicable, the PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffmann-LaRoche, Ltd. For Research Use Only; not for use in diagnostic procedures. Additional Information: GenomeLab™ SNPstream® Genotyping System Specifications and Ordering Information BR-9516B: GenomeLab SNPstream Genotyping Series (PDF Format) GenomeLab SNPstream Genotyping System Parts and Accessories A30456AA: GenomeLab™ SNPstream® Genotyping System Autoprimer Tutorial (PDF Format) A30456AA-App: GenomeLab™ SNPstream® Genotyping System Autoprimer Tutorial--Appendices (PDF Format) Additional GenomeLab™ Information: GenomeLab GeXP Genetic Analysis System GenomeLab SNPstream® Genotyping System Genetic Analysis Applications Genetic Analysis Reagents, Supplies, and Accessories GenomeLab™ Home Page